Site-specific oligonucleotide binding represses transcription of the human c-myc gene in vitro.

Citation:

Cooney, M, et al. “Site-specific oligonucleotide binding represses transcription of the human c-myc gene in vitro.”. Science 241.4864 (1988): , 241, 4864, 456-9. Print.

Date Published:

1988 Jul 22

ISSN:

0036-8075

Abstract:

A 27-base-long DNA oligonucleotide was designed that binds to duplex DNA at a single site within the 5' end of the human c-myc gene, 115 base pairs upstream from the transcription origin P1. On the basis of the physical properties of its bound complex, it was concluded that the oligonucleotide forms a colinear triplex with the duplex binding site. By means of an in vitro assay system, it was possible to show a correlation between triplex formation at -115 base pairs and repression of c-myc transcription. The possibility is discussed that triplex formation (site-specific RNA binding to a DNA duplex) could serve as the basis for an alternative program of gene control in vivo.

Alternate Journal:

Science
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