bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring.

Citation:

Trovisco V, Belaya K, Nashchekin D, Irion U, Sirinakis G, Butler R, Lee JJ, Gavis ER, St Johnston D. bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring. Elife 2016;5

Date Published:

2016 Oct 28

ISSN:

2050-084X

Abstract:

bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot(2A2), which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110-120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.

DOI:

10.7554/eLife.17537

Alternate Journal:

Elife