Structural insights into the tumor-promoting function of the MTDH-SND1 complex.

Citation:

Guo, Feng, et al. “Structural insights into the tumor-promoting function of the MTDH-SND1 complex.”. Cell Rep 86 (2014): , 8, 6, 1704-13. Web.

Date Published:

2014 Sep 25

ISSN:

2211-1247

Abstract:

Metadherin (MTDH) and Staphylococcal nuclease domain containing 1 (SND1) are overexpressed and interact in diverse cancer types. The structural mechanism of their interaction remains unclear. Here, we determined the high-resolution crystal structure of MTDH-SND1 complex, which reveals an 11-residue MTDH peptide motif occupying an extended protein groove between two SN domains (SN1/2), with two MTDH tryptophan residues nestled into two well-defined pockets in SND1. At the opposite side of the MTDH-SND1 binding interface, SND1 possesses long protruding arms and deep surface valleys that are prone to binding with other partners. Despite the simple binding mode, interactions at both tryptophan-binding pockets are important for MTDH and SND1's roles in breast cancer and for SND1 stability under stress. Our study reveals a unique mode of interaction with SN domains that dictates cancer-promoting activity and provides a structural basis for mechanistic understanding of MTDH-SND1-mediated signaling and for exploring therapeutic targeting of this complex.

DOI:

10.1016/j.celrep.2014.08.033

Alternate Journal:

Cell Rep