Capacitance cytometry: measuring biological cells one by one.

Citation:

Sohn, LL, et al. “Capacitance cytometry: measuring biological cells one by one.”. Proc Natl Acad Sci U S A 97.20 (2000): , 97, 20, 10687-90. Web.

Date Published:

2000 Sep 26

ISSN:

0027-8424

Abstract:

Measuring the DNA content of eukaryotic cells is a fundamental task in biology and medicine. We have observed a linear relationship between the DNA content of eukaryotic cells and the change in capacitance that is evoked by the passage of individual cells across a 1-kHz electric field. This relationship is species-independent; consequently, we have developed a microfluidic technique-"capacitance cytometry"-that can be used to quantify the DNA content of single eukaryotic cells and to analyze the cell-cycle kinetics of populations of cells. Comparisons with standard flow cytometry demonstrate the sensitivity of this new technique.

DOI:

10.1073/pnas.200361297

Alternate Journal:

Proc. Natl. Acad. Sci. U.S.A.
Last updated on 01/17/2020