On April 25th, Sarah McFann gave an oral presentation titled “Optogenetic dissection of signaling crosstalk in the early embryo” at The Allied Genetics Conference (ATGC)! Despite the COVID-19 pandemic, the conference proceeded as scheduled (albeit virtually), which allowed Sarah to share her recent work about intracellular Erk dynamics and crosstalk in drosophila embryos.
Says Sarah, "I'm so thankful to the organizers who made the Allied Genetics Conference happen. It was great getting to share my work on signaling crosstalk and dynamics in the fruit fly embryo and being able to hear about research conducted in all sorts of other model organisms--from yeast to mice to wheat."
A fly embryo who recently became famous by appearing in Sarah's presentation.
Congratulations to Alex Goglia on his latest paper titled “A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control” published in Cell Systems!
The study screens a library of over 400 kinase inhibitors for their effect on Erk signaling activity in primary mouse skin cells. Using a live-cell biosensor of Erk activity, the screen explores how different drug treatments can alter unique features of Erk dynamics over time, placing cells into one of three different dynamic regimes, and further uses optogenetic tools to demonstrate that these different regimes affect the conditions under which cells proliferate. As the first live-cell screen of its kind, this work provides a platform for future large-scale screens examining how drugs or other perturbations alter signaling dynamics, which may hold profound consequences for our understanding of inter-pathway signaling crosstalk, cell fate decision making, and disease treatment.
The entire data set from this study can be accessed here.
The Toettchlab warmly welcomes our newest (and cutest) nanobody, Harrison! Although the world is going through a lot of madness right now, we’re glad that Harry and mom, Agi, are doing well. Harry, we’re excited to meet you in person, and we can’t wait to see if you transform into an awesome scientist like your mama someday!
Congratulations to Sidu Jena, who recently presented his research at the joint Keystone Conference in Keystone, CO, on Jan 26-30th! The conference brought together two of Sidu's greatest loves: Gene Regulation and Chromatin Epigenetics.
Sidu's poster was titled: Dynamic signaling and stochastic transcription intersect to modulate heterogeneity.
Says Sidu, "I spent the week learning about super-cool research in gene regulation and chromatin biology, and got the chance to meet and discuss interesting questions and approaches with leaders in the field."
Kicking off 2020, we welcome our newest postdoc, Ellen Reed! Ellen joins us from the University of Pennsylvania, where she completed her thesis work in the lab of Dan Hammer. Her research focused on engineering phase separating intrinsically disordered proteins to respond to external stimuli such as light. Here at Princeton, Ellen will be working as a joint post-doc between the Toettcher lab and the Aoki lab at the National Institute of Natural Sciences in Japan. We’re very excited to have you in lab, Ellen!
On October 28th, our former-undergrad-turned-technician-extraordinaire, Pavithran Ravindran, was invited to give a talk as part of the Trainee Seminar Series at the University of Pennsylvania! Pav was hosted by Dr. Arjun Raj from the departments of Bioengineering and Genetics. His talk was titled “Engineering novel combinatorial and dynamic decoders using synthetic immediate-early genes.” After his talk, he got to spend the day with Arjun and members of the Raj lab.
Says Pav, "Getting the opportunity to prepare a presentation about the work that I have done over the years and visit/present at another university was an incredible experience. I really want to thank both members of the Raj lab/Arjun Raj for hosting me and telling me about the amazing science they are doing as well as everyone in the Toettcher lab for helping me prepare for the talk."
This summer, Sarah McFann spent 12 weeks interning with the quantitative team at the Bill & Melinda Gates Foundation and the creative team at Gates Ventures.
During her internship, Sarah evaluated different mathematical models for how malnutrition leads to developmental stunting. Her goal was to ultimately identify which models could best guide malnutrition interventions. Among the models evaluated were genome-scale metabolic (GEM) models, microbe community GEM models, and ordinary differential equation models, which Sarah explains, “try to capture the complicated relationships between microbial population dynamics in the gut, gut health, and the human immune response.”
In addition to her mathematical work, Sarah says she got to write and learn a lot about the roles that epidemiology, digital health, and renewable energy play in global development.
In describing her experience, Sarah says:
“Working in Seattle was like having a bird’s eye view of the world. I got to see, in real time, how philanthropy can be used to shift markets and how social factors impact epidemics—things you don’t necessarily get to see firsthand in the lab. While it was sobering to realize the bigness of some of the challenges that people around the world are facing right now, it was encouraging to see how many brilliant, empathetic people there are working towards solutions. My summer also got me thinking about ways I can apply the skills I’ve picked up during my PhD to global development problems in health and energy once I graduate.”
We’re very happy to have Sarah back in lab and to have her regale us with tales of her experience and research!
Left to right, Haeun Jung '20, Giselle Uribe '20, Payam Farahani and Qinhao Cao.
This summer, three hardworking undergrads spent ten weeks doing research in the ToettchLab. On August 9th, they each presented their projects at the annual SURP MOL/QCB Poster Session! All three did a great job this summer - visiting student, Qinhao Cao, got working right away with mentor Payam Farahani, and Haeun Jung ’20 and Giselle Uribe ’20 made excellent progress towards their senior theses.
Farewell, Qinhao – it was a pleasure to have you in lab! Haeun and Giselle – we look forward to working with you again when the semester starts!