Light-based feedback for controlling intracellular signaling dynamics.

Citation:

Toettcher, Jared E, et al. “Light-based feedback for controlling intracellular signaling dynamics.”. Nat Methods 810 (2011): , 8, 10, 837-9. Web.

Date Published:

2011 Sep 11

ISSN:

1548-7105

Abstract:

The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.

DOI:

10.1038/nmeth.1700

Alternate Journal:

Nat. Methods