Alignment and calibration of a focal neurotransmitter uncaging system

Citation:

Sarkisov, Dmitry V, and Samuel S-H Wang. “Alignment and calibration of a focal neurotransmitter uncaging system”. Nat Protoc 12 (2006): , 1, 2, 828-32. Web.

Date Published:

2006

ISSN:

1750-2799

Abstract:

Photolysis of caged compounds is a powerful tool for studying subcellular physiological functions. Here we describe protocols for the alignment and calibration of a focal uncaging system. We also report procedures for convenient quantitative calibration of uncaging. Using these methods, we can achieve submicron lateral resolution of photolysis and probe biological function in spines, the smallest signaling compartments of neurons. Initially, the entire alignment procedure takes 4-6 h to perform; periodic fine-tuning of the system takes 1-2 h.

DOI:

10.1038/nprot.2006.124

Alternate Journal:

Nat Protoc