Pif1 family helicases suppress genome instability at G-quadruplex motifs.

Citation:

Paeschke, Katrin, et al. “Pif1 family helicases suppress genome instability at G-quadruplex motifs.”. Nature 497.7450 (2013): , 497, 7450, 458-62. Web.

Date Published:

2013 May 23

ISSN:

1476-4687

Abstract:

The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.

DOI:

10.1038/nature12149

Alternate Journal:

Nature